[ID] CMV colitis in immunocompromised patients(progress; topic review)

[Category] Topic; 1. Workup; 2. Management; 3. Tip and Teaching point; 4. References;

CMV colitis

1.Clinical manifestation.

2.Workup: 
 -How to diagnose?
 -How to evaluate its significance?

3.Management(Indication to start antiviral):

4.How to dose ganciclovir? and anything additional concerning to start meds? 

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[Category] Topic; 1. Workup; 2. Management; 3. Tip and Teaching point; 4. References;

 

CMV colitis

 

1.Clinical manifestation.

Clinical significance: “Active CMV infection” and “CMV disease” are associated with acute allograft failure/death

 

“Active CMV infection: the presence of CMV replication in blood regardless of whether signs or symptoms are present.

 

 “CMV disease” is the presence of detectable CMV in a clinical specimen accompanied by other clinical manifestations. CMV disease may manifest as either CMV syndrome or tissue-invasive CMV disease.

 

In CMV disease,

 

“CMV syndrome”: detectable viral replication in blood with fever, malaise, arthralgia, leukopenia, thrombocytopenia in the absence of tissue-invasive disease.

 

“Tissue-invasive CMV disease” with end-organ disease (enteritis, colitis, hepatitis, nephritis, pneumonitis, meningitis, encephalitis, retinitis; GI, Kidney, Lung, Brain, Eye).

 

Most common Sx = GI disease.

Happens mostly after finishing 3 months of CMV prophylaxis after transplantation.

 

<GI>

Enteritis and/or colitis – Nausea, vomiting, diarrhea, and/or abdominal pain.

Hepatitis with elevated OT/PT with CMV viremia

Pancreatitis with elevated amylase and lipase and CMV viremia.

 

Nephritis – CKD with histologic features of CMV infection in a kidney biopsy specimen.

(Kidney Biopsy)

 

Pneumonitis – Cough, shortness of breath, and pulmonary infiltrates on radiographic imaging plus CMV in BAL

(Bronchoscopy)

 

Meningoencephalitis – Headache, nuchal rigidity, mental status changes, or paralysis, plus CMV in cerebrospinal fluid.

(LP puncture)

 

Retinitis – Retinal edema or hemorrhage

(Ophthalmologist workup)

 

2.Workup: 

 -How to diagnose?

We confirm the diagnosis of CMV infection or disease with nucleic acid testing (NAT).

NAT using the polymerase chain reaction (PCR) for the detection of CMV.

 

Threshold: not clear to differentiate among latent infection, low-level active infection, and CMV disease. Clinical judgment must be used!!(e.g. CMV syndrome: mean 9120 IU; tissue-invasive CMV disease: mean 20,893 IU.)

 

cf. Current COVID-19 test: 1) molecular tests, such as RT-PCR tests(reverse transcription polymerase chain reaction; amplifies many copies of a segment of the “N” gene by using primer and DNA polymerase; 30-45 cycles of PCR needed to be detected.), that detect the virus's genetic material, 2) antigen tests that detect specific proteins on the surface of the virus.

 

-What about serology test? such as IgG, IgM

Enzyme immunoassays(EIA) and indirect and anticomplement immunofluorescence(ACIF) assays are the most commonly used.(cf. Enzyme-Linked Immunosorbent Assay = ELISA)  

-          Recent and Acute infection: CMV-specific IgM Ab(+); CMV-specific IgM antibodies are typically detectable within the first two weeks after the development of symptoms and may persist for several months. Among patients with acute CMV infection who had serial serum samples available for analysis, CMV-specific IgM antibodies were detectable for a period of four to six months after the onset of symptoms. Therefore, a positive CMV-specific IgM antibody alone may provide misleading information if a prior baseline test is not available (in cases in which it represents prior infection rather than current infection).

-          X4 or more increase in CMV-specific IgG titers in paired specimens obtained at least 2 weeks apart; CMV-specific IgG antibodies are often not detectable until two to three weeks following the onset of symptoms and persist lifelong 

 

 -How to evaluate its significance?

   Sx.

   and/or Organ involvement(more significant consequence)

 

3.Management(Indication to start antiviral):

 Active CMV infection vs CMV disease

 Among transplant recipients who have active CMV infection but who are asymptomatic, we stop the antimetabolite immunosuppressant (ie, azathioprine or mycophenolate). We do not use antiviral treatment in such patients, unless they develop symptoms or viremia does not resolve with stopping the antimetabolite. However, many centers add an antiviral agent upon recognition of CMV reactivation, especially in high-risk patients. Decision points to dictate aggressiveness in this setting should include consideration of dose of immunosuppression or cumulative exposure to immunosuppression and whether the patient is CMV-seropositive (CMV R+) or seronegative (CMV R-) as the latter group has a higher risk for rapid development of disease. (See 'Active CMV infection' above.)

Among all transplant recipients who have CMV disease (either CMV syndrome or tissue-invasive disease), we initially stop the antimetabolite immunosuppressant (ie, azathioprine or mycophenolate) and provide antiviral treatment. Our selection of agent depends on the severity of the clinical manifestations and the level of viremia and, among some patients, the pattern of drug resistance. (See 'CMV disease' above.)

Among all patients with life-threatening CMV disease (eg, pneumonitis, meningoencephalitis), high viral loads, or moderate to severe gastrointestinal disease, we recommend full treatment doses of antiviral therapy with intravenous (IV) ganciclovir rather than oral valganciclovir (Grade 1B). Ganciclovir has been shown to be effective among kidney transplant patients with severe CMV infections. The dose of ganciclovir is 5 mg/kg IV every 12 hours (adjusted for kidney function).

Among patients with mild CMV disease (ie, those with minimal signs and symptoms) who are expected to have good absorption of oral medications, we recommend full treatment doses of oral valganciclovir rather than IV ganciclovir (Grade 1B). Oral valganciclovir has been shown to be as effective as ganciclovir in patients with mild to moderate CMV infection and spares patients from the risks and cost of central venous access. The dose of valganciclovir is 900 mg orally twice daily (adjusted for kidney function). (See 'Antiviral therapy' above.)

We generally continue one of the antiviral regimens at the full treatment doses described above until the clinical signs and symptoms of CMV disease have completely resolved and there is no evidence of CMV viremia in two blood PCRs performed at least one week apart. The typical duration of full treatment doses of antiviral therapy is 21 days but can range from 14 to 28 days or longer. Once symptoms and viremia have resolved, we treat all patients with a one- to three-month course of oral valganciclovir at 900 mg once daily (adjusted for kidney function) to prevent relapse; this reduced dose of valganciclovir is sometimes referred to as secondary prophylaxis. (See 'Duration of therapy' above.)

Ganciclovir resistance has become more common with the institution of preventive treatment of CMV. Patients with ganciclovir-resistant CMV may require a higher dose of ganciclovir, or IV foscarnet, depending on the identified genotype. Patients with life-threatening disease (such as CMV pneumonitis) that progresses despite antiviral agents and reduction of immunosuppression agents may be treated with cytomegalovirus immune globulin (CytoGam, CMV Ig) or intravenous immune globulin (IVIG) irrespective of the mutation that is identified and if no mutation is identified. (See 'Drug-resistant CMV' above.)

 

-4.How to dose ganciclovir? and anything additional concerning to start meds? 

 - monitor (efficacy vs adverse event) 

Px.

CMV disease increases allograft loss and mortality:

In a single-center study of 51 CMV D+/R- patients who developed CMV disease after stopping antiviral prophylaxis (49 percent with CMV syndrome and 51 percent with tissue-invasive CMV disease), CMV disease was associated with a 2.8-fold increased risk of allograft loss or death, whereas CMV syndrome was not [2].

In a multicenter study of 15,848 United States kidney transplant recipients assembled using large administrative data, CMV disease occurring >100 days posttransplant was identified in 4 percent of patients, and CMV disease occurring <100 days posttransplant was identified in 1.2 percent of patients. In multivariable analysis, CMV disease occurring 101 to 365 days posttransplant, and CMV disease occurring >365 days posttransplant were associated with a 1.5- and 2.1-fold increased risk of death, respectivel

 

[REF: Uptodate; https://www.uptodate.com/contents/kidney-transplantation-in-adults-clinical-manifestations-diagnosis-and-management-of-cytomegalovirus-disease-in-kidney-transplant-recipients?search=cmv%20diarrhea&source=search_result&selectedTitle=4~150&usage_type=default&display_rank=4]

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